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Monash Micro Imaging Equipment

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Optical Microscopy

  1. Olympus Provis Ax70 microscope for brightfield, darkfield, fluorescence and polarisation microscopy. With B/w and colour digital cameras (Sis FVII and Olympus DP70), and SiS AnalySis software.
  2. Olympus BX60 microscope with Colour camera (Sis CVII), for transmitted light, reflected light, reflected polarisation and fluorescence.
  3. A variety of other microscopes: Stereo dissecting microscope with digital camera, fluorescence microscope, DIC, polarisation microscope etc.
  4. Leica AF6000LX Live Cell Imaging workstation, fully motorised with CO2 incubator, good range of lenses, stage adaptors for all types of chambers and microplates, software for 3D and multidimensional microscopy, FRET, all kinetic imaging, cell tracking and deconvolution. Independent offline workstation with full software included.
  5. Olympus .slide System (with BX51 Microscope), motorised stage with inserts for 4 regular slide or large format slide, lenses from 2-60x for brightfield and fluorescence (U-MNIBA3, U-WIG3, U-MWU2), Peltier-cooled high sensitivity camera (colour) for BF and Fl, and dotSlide software for analysis, and tele-(virtual)microscopy.

Confocal Microscopy

  1. Leica TCS NT (upright microscope), 3 channel fluorescence imaging, plus transmitted light.
  2. Leica TCS NT (invert microscope), 3 channel fluorescence imaging, plus transmitted light, live cell imaging.
  3. Yokogawa spinning-disc confocal with Andor Ixon EMCCD camera and IQ software for live cell imaging. Full range of high NA lenses, objective focuser, temperature controlled observation chamber, and analysis software.
  4. Olympus FV1000 spectral confocal microscope, with full range of lenses, and temperature controlled observation chamber for live cell imaging.
  5. Leica SP Multiphoton Microscope (based on DMI6000 Invert microscope), with Spectraphysics MaiTaiHP (3W TiSa pulsed laser) for live cell and tissue imaging.
  6. Leica SP Multiphoton Microscope (based on DM6000 upright fixed stage microscope), with Spectraphysics MaiTaiHP (3W TiSa pulsed laser), for live animal imaging [housed at animal house at Monash Health Research Precinct ].

Electron Microscopy

Transmission EM

  1. Hitachi H7500 TEM, 120 KV Microscope with High contrast and High resolution imaging capabilities, includes digital camera (Gatan) – Building 13C
  2. 200EX Jeol 200KV TEM (Building 17, Rm. B23)
  3. 2 x Jeol 100S 100KV TEM’s with large format photographic imaging for general imaging.
  4. Cryo tissue preparation equipment including Leica slam and plunger tissue freezing unit, 2x Leica freeze substitution units and 2x Leica cryo ultramicrotomes (Building 13C)
  5. Ultramicrotomy: 5x ultramicrotomes (Building 13C and 17) – various ages.

Scanning EM

  1. Hitachi S570 SEM (B16) - With high resolution in-lens secondary electron detector, back scatter detector, cathodoluminescence detector & digital imaging.
  2. Emscope SP2000 (B16) -For cryo-preparation and cryo-SEM of delicate samples or those significantly affected by conventional preparation techniques
  3. Ancillary specimen preparation equipment:
    • Balzers SCD005 Sputter coater (B23) - For gold coating of SEM samples.
    • Dynavac Evaporative Coater (B23) - For carbon coating of SEM samples.
    • Balzers CPD003 Critical Point Dryer (B21) - For routine preparation of SEM samples
    • Dynavac FDC/H Freeze Dryer (B21) - For preparation of SEM samples

Other Support Equipment and Labs

  1. General tissue processing laboratories, There are two tissue-processing laboratories. One is located in Building 13C and the other in Building 17. Each laboratory is fully equipped and stocked with the chemicals required for preparing tissues for different applications.
  2. Photographic darkrooms with both film processing facilities and paper printing with a Durst Laboro enlarger (Building 13C and 17)
  3. Digital imaging/analysis lab
    Computers:
  • Image server with RAID storage
  • 4 high end PCs with 2-4GB RAM
  • Dedicated Leica analysis workstation running LAS for live cell analysis.

High resolution film and image scanner: Imacon Flextight Precision III, with Speed up to 20 Mb per min, Resolution 6300 dpi, true 14 bit colour-4.2 Dmax in a single pass Scanning, SCSI interface.

Printer: Tetronix Phaser A4 and A3 high-resolution colour and black and white printer.

Software:

  • AnalySis (Olympus Soft Imaging) software for EM, LM, fluorescence, confocal and multidimensional analysis.
  • 3D Imaging: 3D-doctor, Eikona 3D, Voxblast, Imaris.
  • Offline processing of confocal data: Leica LASAF (for SP5 and LX data), Analysis (for Fluoview and other data), IQ (for spinning disk data), etc
  • Photoshop – graphic processing
  • Image viewers available for all microscopy formats (Olympus, Leica, SiS, Zeiss)